Master’s Thesis Grants | JAE Intro ICUs 2023

Supervisors: Juan José Rodríguez Herrera (juanherrera@iim.csic.es), José Manuel Fernández Babarro (jbabarro@iim.csic.es) e Alejandro Garrido Maestu (alejandro.garrido@inl.int)

Grupos: : Microbioloxía and Tecnoloxía of Produtos Marine & Ecofisioloxía, Biomarcadores and Management Sostible of Bivalves.

Code: IIM-16

The verteduras of augas residuais provocan the bioaccumulation of pathogens and contaminantes in bivalves, podendo causar problems of seguridade food. Conseguintemente, the zonas of farming are suxeitas the vixilancia sanitaria by mostraxe of the own mollusks, but isto with leva serias limitacións asociadas to the baixa frecuencia with that realizan. In change, a monitoring continua of the carga microbiana contaminante not environment circundante permitiría predicir eventos of contaminación and salvagardar the seguridade food. With this obxecto proponse a proba concepto dirixida the vincular the contaminación fecal not environment marine and in bivalves.

The persoa bolseira participaría in the characterization of the dinámicas of bioaccumulation and eliminación of Escherichia coli in mussels, in escenarios that simulen vertidos of augas residuais. For iso realizaranse ensaios experimental on a instalación piloto us that dosificará esa bacteria. The parámetros environmental monitorizaranse of modo continuo and, periodicamente, analizaranse microbiolóxicamente the animais and the water in the that atopan, así as the response fisiolóxica and metabólica of the mussels. In addition, desenvolverase a método for monitorizar E. coli baseado in the degradación dun substrato específico of this bacteria, enfocando their optimization for reducir the tempo of detección for poder meet the objective of monitoring in continuo.

Isto supoñeralle a first aproximación to the microbioloxía ambiental and food, by a banda, and to the fisioloxía of bivalves, by outro, adquirindo knowledge in design of experimentos, discusión scientific and the funcionamento of instalacións experimental the escala piloto.

Requirements: Applicants must be in posesión of the Bachelor’s degree or Degree in Sciences Biolóxicas, marine sciences or Biotechnology, and not must start the Master’s Thesis project.

GPA minimum (Grade Average of Bachelor’s degree on 10): 8.00

Supervisors: José Manuel Fernández Babarro (jbabarro@iim.csic.es) , Laura G. Peteiro (lpeteiro@iim.csic.es) e Miguel Gil Coto (mgilcoto@iim.csic.es)

Grupos:Ecofisioloxía, Biomarcadores and Management Sostible of Bivalves & Ocean Processes in Global Change  

Code: IIM-17

The reprodución of the mussel is a evento natural and necessary of expulsión of gametos sexuais (masculinos and femininos) to the environment for their posterior fecundación. The process of desova represents a momento of vulnerabilidade, with the exposición of tecidos brandos the predadores, but is a process fisiolóxico natural and periódico. Of this capacity reprodutiva depende the abastecemento of the semente of mussel in the franxa litoral and/the in colectores situados in the own batea of farming onde fixan the larvas despois of their life peláxica.

Na actualidade, existen sistemas de monitoring of high frecuencia and not invasivos (chamado, polas súas siglas in English, HFNI) that allow caracterizar the ciclos of apertura-peche valvar of the bivalves with large detail as indicador of the behavior in relación the numerosas funcións vitais of the body (fisioloxía) así as respecto the changes abióticos and biolóxicos of the environment.

O desenvolvemento inicial deste monitoring permitiu determine that the mussel in situación of farming mantén their valvas abertas the greater parte of the tempo (98%) yet habendo a elevada variability not environment abiótico (temperatura, salinidade, dispoñibilidade of food…).

The work desenvolvido in this project aims to caracterizar the behavior valvar of the mussel durante the eventos of desova cun sinal específico that permita usar this tecnoloxía as system of alerta temperá, así as determine the incidencia of environmental factors clave (temperatura and dispoñibilidade of food) in the reprodución and the desova, and possible disrupcións in this process reprodutivo. Disrupcións that can affect to the eficiencia of the reprodución and of the desova, así as to the posterior recrutamento of populations naturais by parte of the industria acuícola.

Requirements: Applicants must be in posesión of the Bachelor’s degree or Degree in marine sciences, Biology or Sciences Environmental. Not must start their Master’s Thesis project (TFM)..

GPA minimum (Grade Average of Bachelor’s degree on 10): 8.00

Supervisors: Josep Rotllant Moragas (rotllant@iim.csic.es) and Xosé Antón Vázquez Álvarez (jvazquez@iim.csic.es)

Grupos: Laboratorio of Aquatic Biotechnology & Reciclado and Valorización of Residuos

Code: IIM-18

The objective deste work is evaluate the uso of hidrolizados encimáticos of proteínas (FPH) obtidos the from of refugallos and subprodutos of peixe as novos ingredientes for substituír the fariña of peixe and poder utilizalos in the formulación of pensos more sostibles and eficientes dirixidos to the farming intensivo of the main species of fish as are the rodaballo, the robaliza or the dourada.

The FPH recentemente comezaron the usarse for improve the crecemento of different species of fish with resultados notables, but yet not produciron, probado and avaliado cun enfoque multidisciplinar that inclúe the application of technicians fisiolóxicas, engineering chemical, bioquímica, biology molecular and technicians metagenómicas of final xeración.

The enfoque that is desenvolvendo from the IIM, in colaboración with the UVigo, IEO-CSIC and the USC, inclúe the produción of the FPH and the response integrada of the farming of rodaballo the these hidrolizados, baseándose in aspectos as the apetencia, the perfís nutricionais, the crecemento animal, the integridade of the tracto dixestivo and the characterization of the microbiota intestinal.

Requirements: Applicants must be in posesión of the Bachelor’s degree or Degree in Biology, Biotechnology, Chemical, Bioquímica or Engineering and not must haber started the Master’s Thesis project.

GPA minimum (Grade Average of Bachelor’s degree on 10): 7.00

Supervisors: Josep Rotllant (rotllant@iim.csic.es) e Miguel Planas (mplanas@iim.csic.es)

Grupos: Laboratorio of Aquatic Biotechnology & Ecology and Marine Resources.

Code: IIM-19

The animais and the plantas are, in mesmos, hábitats for many comunidades microbianas. These hábitats are temporais and sofren changes dratticos cando these hóspedes morren. Cando the microbios asócianse with hóspedes vivos have acceso privilexiado to the materia orgánica of the host falecido, the that can levar the that the microbios adopten estilos of life oportunistas and adaptables.

In this study, investigaremos the dynamic temporal of the microbiota of the common octopus, rastrexando the changes in their community microbiana durante the period of senescencia of the octopus, cando the femias morren pouco despois of the apareamento. For comprender this process, is necessary comprender the genetic diversity, fisiolóxica, metabólica and especialmente ambiental impartida and compartida polos microorganisms that cohabitan.

The diversidade espacial and temporal substancial of the microbiomas animais not só is dynamic senón the miúdo predicible, the that the converte in indicadores potenciais of varios factores intrínsecos and extrínsecos. These microorganisms inflúen, by a banda, in the predisposición to the health and the enfermidade and, doutra banda, xogan a role fundamental not process of descomposición.

By both, the hypothesis that will explore in this project is that the period that surrounds the death of the host plays a role important in the ecology of the microbiota of the host and in the evolution both of the host as of their microorganisms.

Requirements: Applicants must be in posesión of the Bachelor’s degree or Degree in Biology, Biotechnology, Chemical, Bioquímica, marine sciences or Engineering and not must start the Master’s Thesis project.

GPA minimum (Grade Average of Bachelor’s degree on 10): 7.00.

Supervisors: Eva Balsa Canto (ebalsa@iim.csic.es), Antonio Figueras (antoniofigueras@iim.csic.es) y Beatriz Novoa (beatriznovoa@iim.csic.es)

Grupos: Biosistemas and Engineering of Bioprocesos & Immunology and Genomics.

Code: IIM-20

The hemocitos are presentes in all the cavidades of the invertebrates marine by the that circula the hemolinfa. The hemocitos desempeñan funciones both in the procesos fisiológicos (p. ej., the digestión and the training of the cubierta) as in the funciones inmunitarias (p. ej., the fagocitosis, the síntesis of efectores inmunitarios and the modulación of the respuestas inmunitarias), until punto in that the hemolinfa and the hemocitos internos of bivalves are casi libres of bacterias.

In mussel hemos descrito cómo the hemocitos can migrar the the espacios intervalvares that son esencialmente agua de mar¹. In this environment delimitado by the conchas, habitan bacterias and otros pequeños eucariotas. Usaremos modelos matemáticos for descifrar the dynamic of the interacciones of the bacterias with the células of the mussel in the líquido intervalvar and the hemolinfa.

The modeling abordará the level of población, utilizando modelos ecológicos for identificar tipos of interacciones (cooperativas the antagónicas). Proponemos identificar the miembros centrales of the microbioma and construiremos modelos matemáticos ecológicos (tipo Lotka-Volterra generalizado) for contextualizar the data metagenómicos obtenidos in laboratorio in diversas conditions.

As resultado, obtendremos information preliminar on the tipos of interacciones of the species bacterial seleccionadas and the host. All ello conducirá the desarrollos posteriores in the that abordaremos modelos metabólicos the escala genómica.

(1) Panebianco, A. et al. Mytilus galloprovincialis releases immunologically functional haemocytes to the intervalvar space in response to tissue injury and infection. Fish Shellfish Immunol. 138, 108806 (2023).

Requirements: Applicants must be in posesión of the Bachelor’s degree the Degree in Biotechnology, Engineering Biológica the Engineering of Bioprocesos and not must haber started the Master’s Thesis project.

GPA minimum (Grade Average of Bachelor’s degree on 10): 7.50

Supervisors: Miriam García Rodríguez (miriamr@iim.csic.es) and Xosé Antón Vázquez Álvarez (jvazquez@iim.csic.es) .

Grupos: Biosistemas and Enxeñaría of Bioprocesos  & Reciclado and Valorización of Residuos, 

Code: IIM-21

This work aims to develop tools both experimental as theoretical that us allow improve the knowledge of the resistance bacterial the compounds of different structure chemical. Using bacterias pathogenic surrogate of the industria food, as E. coli, traballarase in the execución of experiencias of inactivación bacteriana and of selection of estirpes bacterial persistentes the different biocidas and antibióticos.

For iso ensaiaranse cultures of inhibición of the crecemento bacteriano the eses tóxicos: Operarase both in modo descontinuo, the escalas of microplaca and matraz, as in modo continuo, utilizando a morbidostato deseñado especificamente for this propósito. Cuantificarase the response bacteriana mediante densidade óptica, cálculo of colonias viables in placas and determination of mortalidades and tamaños celulares mediante citometría of fluxo.

In addition, levará the cabo a modelando matematicamente of the respostas with the fin of establecer protocolos óptimos of desinfección not ámbito of the industria food. In this final caso preténdese the incorporation of tales tools mathematics nun software “amigable” of design own and their validation in the formulación of xemelgos dixitais of interest industrial in procesos of descontaminación bacteriana.

Requirements: Applicants must be in posesión of the Bachelor’s degree or Degree in Biotechnology, Engineering Chemical, Chemical or Bioquímica and not must start the Work of Fin Matter.

GPA minimum (Grade Average of Bachelor’s degree on 10): 7.00

Supervisors: Ángel F. González (afg@iim.csic.es) and Xosé Antón Álvarez Salgado (xsalgado@iim.csic.es)

Grupos: Ecology and Marine Resources & Laboratorio of Xeoquímica Orgánica.

Code: IIM-22

The factores responsables of the éxito of the recrutamento us cephalopods dependen principalmente of the interaccións between the fases of masa of ovos, subadultos recentemente nados and pre-recrutados and the environment physics and biolóxica that prevalece durante each fase. By both, débese salientar the censos of paralarvas, already that are fundamentais for study the distribución, biology, áreas of desova and structure poboacional of the cephalopods. With all, the samples relativamente pequenas obtidas and the súas distribucións irregulares seguían sendo the main limitacións us studies of paralarvas silvestres.

The finais of the 90 and principios of the 2000, the studies directos of paralarvas planctónicas and their response to the variation oceanográfica eran escasos in comparación with outras larvas of invertebrates and fish. The studies more completos of the historia of life temperá of the cephalopods levaron the cabo in augas xaponesas, and not Atlántico noroccidental, onde find the greater abundancia of species planctónicas. From entón, the studies realizados in Galicia supuxeron a of the main achegas worldwide on the understanding of the relación between the parámetros oceanográficos and the larvas of cephalopods, especialmente Octopus vulgaris.

 Durante the dúas últimas décadas lideramos seven projects multidisciplinares that proporcionaron the primeiros data of the marcos meteorolóxicos, físicos and bioxeoquímicos not hábitat of the paralarvas of cephalopods, on a zona of importancia pesqueira (caladoiro of the Illas Cíes, fronte to the Vigo Ría). The mostraxes oceanográficas and biolóxicas realizados durante these projects realizáronse in colaboración with numerosos researchers nacionais and internacionais, the that permitiu desentrañar algúns aspectos of the relación between the primeiras etapas of life of the cephalopods and the parámetros físicos, químicos and biolóxicos on a of the main rexións pesqueiras of Europe. A of the pezas for complete within of the ecology of the octopus is the fase previous to the asentamento of the larvas of octopus and their marco oceanográfico.

The persoa for incorporar encargarase of analyze and correlacionar the abundancia and of the samples of octopus and of zooplancton obtidas durante the campaña ECOSUMA the bordo of the Sarmiento of Gamboa, included the parasitos that atopen nelas, with the conditions oceanográficas that prevaleceron durante the period of study. By outra banda, contribuirá to the biobancarización destas samples and their data asociados.

Requirements: Applicants must of be in posesión of the Bachelor’s degree in Biology or of the Bachelor’s degree in marine sciences.

GPA minimum (Grade Average of Bachelor’s degree on 10): 7.50